Herbal medicine is increasingly being used as a safe and effective alternative treatment. One plant that has antibacterial potential against antibiotic-resistant Extended Spectrum Beta-Lactamase (ESBL)-producing Escherichia coli bacteria is the leaves of bangun-bangun (Plectranthus amboinicus (Lour)). Research shows that this leaves extract effectively inhibits and kills the growth of ESBL-producing Escherichia coli. This study aims to explore more deeply the antibacterial activity of bangun-bangun leaves, with the hope of supporting the development of herbal medicine and improving public health. This research is an in vitro descriptive experimental research in the laboratory. The test groups used are several concentrations of ethanol extract of bangun-bangun leaves, namely, 2000 μg/mL, 1000 μg/mL, 500 μg/mL, 250 μg/mL, and 125 μg/mL with 4 repetitions. This study was conducted using the dilution method to determine the minimum inhibitory concentration (MIC) and minimum kill concentration (MBC).  The results of this study showed that bangun-bangun leaves extract with a concentration of 1000 μg/mL as the minimum kill concentration (MBC) and 500 μg/mL as the minimum inhibitory concentration (MIC). The extract of bangun-bangun leaves has antibacterial activity against ESBL-producing Escherichia coli bacteria.